MersV1, France, Analysis, bibRecord, 000385

Polymorphism in N-2-acetylaminofluorene induced DNA structure as revealed by DNase I footprinting.

Identifieur interne : 000385 ( France/Analysis ); précédent : 000384; suivant : 000386

Polymorphism in N-2-acetylaminofluorene induced DNA structure as revealed by DNase I footprinting.

Auteurs : X. Veaute [France] ; R P Fuchs

Source :

Nucleic acids research [ 0305-1048 ] ; 1991.

RBID : pubmed:1945836

Descripteurs français

KwdFr :
- ADN (), Altération de l'ADN, Conformation d'acide nucléique, Deoxyribonuclease I (métabolisme), Données de séquences moléculaires, Mutation, N-Fluorén-2-yl-acétamide (), Polymorphisme génétique, Séquence nucléotidique.
MESH :
- métabolisme : Deoxyribonuclease I.
- ADN, Altération de l'ADN, Conformation d'acide nucléique, Données de séquences moléculaires, Mutation, N-Fluorén-2-yl-acétamide, Polymorphisme génétique, Séquence nucléotidique.

English descriptors

KwdEn :
- 2-Acetylaminofluorene (chemistry), Base Sequence, DNA (chemistry), DNA Damage, Deoxyribonuclease I (metabolism), Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Polymorphism, Genetic.
MESH :
- chemical , chemistry : 2-Acetylaminofluorene, DNA.
- chemical , metabolism : Deoxyribonuclease I.
- Base Sequence, DNA Damage, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Polymorphism, Genetic.

Abstract

In this paper, we have constructed double stranded helices (60-mers) containing a single N-2-acetylaminofluorene (-AAF) adduct covalently bound to one of the three guanine residues of the Narl site (G1G2CG3CC). This sequence was identified as a strong frameshift mutation hot spot for many carcinogens that bind to the C8 position of guanine. Using DNase I as a probe for DNA conformation we show i) that the average size of the helix deformation extends over 3 to 5 base pairs in both directions from the adduct site, and ii) that there is a strong polymorphism in the adduct induced DNA conformation. The present study supports the idea that adducts induce specific sequence dependent local conformational changes in DNA that are differentially recognized and processed by the enzymatic machineries that lead to repair or mutagenesis.

DOI: 10.1093/nar/19.20.5603
PubMed: 1945836

Affiliations:

Links to Exploration step

pubmed:1945836

Le document en format XML

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<front><div type="abstract" xml:lang="en">In this paper, we have constructed double stranded helices (60-mers) containing a single N-2-acetylaminofluorene (-AAF) adduct covalently bound to one of the three guanine residues of the Narl site (G1G2CG3CC). This sequence was identified as a strong frameshift mutation hot spot for many carcinogens that bind to the C8 position of guanine. Using DNase I as a probe for DNA conformation we show i) that the average size of the helix deformation extends over 3 to 5 base pairs in both directions from the adduct site, and ii) that there is a strong polymorphism in the adduct induced DNA conformation. The present study supports the idea that adducts induce specific sequence dependent local conformational changes in DNA that are differentially recognized and processed by the enzymatic machineries that lead to repair or mutagenesis.</div>
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Serveur d'exploration MERS

Polymorphism in N-2-acetylaminofluorene induced DNA structure as revealed by DNase I footprinting.

Polymorphism in N-2-acetylaminofluorene induced DNA structure as revealed by DNase I footprinting.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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